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Publication Date: 2011-07-01
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Henao Uribe, F. J., Valencia Giraldo, J. A., Díaz Franco, O., & Rangel Sierra, M. Y. (2011). Effects of adition of se minal plasma on the elimination of cytoplasmic droplets in of sus Scrofa Linaeus, 1758 boar semen. Boletín Científico. Centro De Museos, 15(2), 94–104. Retrieved from https://ucaldas.metarevistas.org/index.php/boletincientifico/article/view/4611
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Abstract
The cytoplasmic droplets (CDs) are remnants of the cytoplasm that are attached to the sperm after spermatogenesis. CDs constitute the most frequent sperm abnormality in pigs and are clearly related to low fertility. There are serious indications that fructose and the seminal plasma AMPc are involved in sperm maturation in the CDs detachment and in the acrosome reaction. The objective of this study was to evaluate the effect of the addition of seminal plasma in the CDs detachment in males with diagnosis of persistence of such detachment. Three boars (two with persistence of CDs and one normal) from three to five years of age, housed in the Montelindo farm at Universidad de Caldas were used in the study. These boars were performed complete seminal analysis weekly during four months. A factorial arrangement 3x3x2 (addition to the males FR with CDs persistence of 0%, 20% of SPHM and 20% of SPMCDs; 0, 60 and 120 minutes incubation, and 16, and 37°C incubation temperature) was carried out in a randomized complete blocks design, analyzed through variance analysis and Tukey's test. Incubation of males semen with persistence of CDs with SPMCDs decreased more than 4% the CDS with regards to incubation without SP and SPHM; similarly, there was reduction of approximately 5% in CDs when increasing incubation time from 0 to 120 minutes, and about 2% when increasing incubation temperature from 16 to 37°C.
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References
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BERGER, T. & CLEGG, E.D., 1985. Effect of male accessory gland secretions on sensitivity of porcine sperm acrosomes to cold shock, initiation of motility and loss of cytoplasmic droplets. J Anim Sci., 60: 1295-1302.
BONET, S., BRIZ, M. & FRADERA, A., 1993. Ultrastructural abnormalities of boar spermatozoa. Theriogenology, 40: 383-396.
CABALLERO, I., 2007. Estudio del plasma seminal y la espermadhesina PSP-I/PSP-II sobre la funcionalidad de los espermatozoides de verraco: Tesis, Universidad de Murcia, Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, España.
CABALLERO, I., VÁZQUEZ, J.M., GIL. M.A., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M., RODRÍGUEZ-MARTÍNEZ, H. & MARTÍNEZ, E.A., 2004. Does seminal plasma psp-i/psp-ii spermadhes in modulate the ability of boar spermatozoa to penetrate homologous oocytes in vitro? J Androl., 25: 1004-1012.
CALVETE, J.J., DOSTÁLOVÁ, Z., SANZ, L., ADERMANN, K., THOLE, H.H., TÖPFER-PETERSEN, E., 1996. Mapping the heparin-binding domain of boar spermadhesins. FEBS Letters, 379: 207-211.
CALVETE, J.J., ENSSLIN, M., MBURU, J., IBORRA, A., MARTÍNEZ, A., ADERMANN, K., WABERSKI, D., SANZ, L., TÖPFER-PETERSEN, E., WEITZE, K.F., EINARSSON, S. & RODRÍGUEZ-MARTÍNEZ, E., 1997. Monoclonal antibodies against boar sperm zonapellucida-binding protein awn-1. Characterization of a continuous antigenic determinant and immunolocalization of awn epitopes in inseminated sows. Biol Reprod., 57: 735-742.
CENTURIÓN, F., VÁZQUEZ, J.M., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M. & MARTÍNEZ, E.A., 2003. Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution. Biol Reprod., 69: 640-646.
EKHLASI-HUNDRIESER, M., SINOWATZ, F., GREISER DE WILKE, I., WABERSKI D. & TÖPFER-PETERSEN, E., 2002. Expression of spermadhesin genes in porcine male and female reproductive tracts. Mol Reprod Dev., 61 (1): 32-41.
FISCHER, K.A., LEYEN, K.V., LOVERCAMP, K.W., MANANDHAR, G., SUTOVSKY, M., FENG, D., SAFRANSKI, T. & SUTOVSKY, P., 2005. 15-lipoxygenase is a component of the mammalian sperm cytoplasmic droplet. Reproduction, 130: 213-222.
FOLEY, C.W., HEIDENREICH, C.J., HARRINGTON, R.B., JONES, H.W. & ERB, R.E., 1964. Changes in fructose, lacticacid, ph, and motility of boar semen during incubation at 37°C. J Anim Sci., 23: 558-561.
GARCÍA, E.M., 2007. Análisis, función y aplicaciones biotecnológicas de las proteínas del plasma seminal de porcino PSP I y PSP II: Tesis, Universidad de Murcia, Facultad de Veterinaria, Departamento de Medicina y Cirugía Animal, España.
GARCÍA, J.C., DOMÍNGUEZ, J.C., PEÑA, F.J., ALEGRE, B., GONZÁLEZ, R., CASTRO, M.J., HABING, G.G. & KIRKWOOD, R.N., 2010. Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility. Anim Reprod Sci., 119: 160-165.
GARNER, D.L., THOMAS, C.A., GRAVANCE, C.G., MARSHALL C.E., DEJARNETTE, J.M. & ALLEN, C.H., 2001. Seminal plasma addition attenuates the diltion effect in bovine sperm. Theriogenology, 56: 31-40.
GÓMEZ, G., 2010. Dinámica de la calidad seminal de verracos del centro-occidente colombiano: Tesis, Universidad de Caldas, Maestría en Sistemas de Producción Agropecuaria, Manizales, Colombia.
HARAYAMA, H., SHIBUKAWA, T., MIYAKE, M., KANNAN, Y. & KATO, S., 1996. Fructose stimulates shedding of cytoplasmic droplets from epididymal boar spermatozoa. Reprod Fertil Dev., 8: 1039-1043.
JONÁKOVÁ, V. & TICHÁ, M., 2004. Boar seminal plasma proteins and their binding properties. A review Collection of Czechoslovak Chemical Communications, 69: 461-475.
KAPLAN, M., RUSSELL, L.D., PETERSON, R.N. & MARTAN, J., 1984. Boar sperm cytoplasmic droplets: Their ultrastructure, their numbers in the epididymis and at ejaculation and their removal during isolation of sperm plasma membranes. Tissue and Cell, 16: 455-468.
KATO, S., SHIBUKA, W.A.T., HARAYAMA, H., et al., 1996. Timing of shedding and dissentegration of cytoplasmic droplet from boar and goat spermatozoa. J. Reprod. Dev., 42: 237-241.
KAWANO, N., SHIMADA, M. & TERADA, T., 2004. Motility and penetration competence of frozen-thawed miniature pig spermatozoa are substantially altered by exposure to seminal plasma before freezing. Theriogenology, 61: 351-364.
LOVERCAMP, K.W., SAFRANSKY, T.J. & FISCHER, K.A., 2007. High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters. Arch Androl, 53: 219-228.
OKAMURA, N., DACHEUX, F., VENIEN, A., ONOE, S., HUET, J.C. & DACHEUX, J.L., 1992. Localization of a Maturation-Dependent Epididymal Sperm Surface Antigen Recognized by a Monoclonal Antibody Raised against a 135-Kilodalton Protein in Porcine Epididymal Fluid. Biol Reprod., 47: 1040-1052.
PÉREZ-LLANO, B., LORENZO, J.L., YENES, P., TREJO, A. & GARCÍA-CASADO, P. 2001. Short hypoosmotic swelling test for the prediction of boar sperm fertility. Theriogenology, 56: 387-398.
PURSEL, V.G. & JOHNSON, L.A., 1974. Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Theriogenology, 1: 63-68.
RODRÍGUEZ-MARTÍNEZ, H., SARAVIA, F., WALLGREN, M., TIENTHAI, P., JOHANNISSON, A., VÁZQUEZ, J.M., MARTÍNEZ, E., ROCA, J., SANZ, L. & CALVETE, J.J., 2005. Boar spermatozoa in the oviduct. Theriogenology, 63:514-535.
ROZEBOOM, K.J., 2000. Evaluating of boar semen quality (en) N. C. S. UNIVERSITY (ed.) Animal Science Facts. Extension Swine Husbandry, North Carolina State, 1-8.
SALA-ECHAVE, R., REGUERA, G., GARCÍA-CASADO, P. & PÉREZ-LLANO, B., 2008. Influencia del calentamiento de las dosis seminales porcinas sobre la calidad espermática durante la I.A. Av. Tecnol. porc., 5 (3): 34-41.
SARAVIA, F., WALLGREN, M., JOHANNISSON, A., CALVETE, J.J., SANZ, L., PEÑA, F.J., ROCA, J. & RODRÍGUEZ-MARTÍNEZ, H., 2009. Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks. Theriogenology, 71: 662-675.
WABERSKI, D., SÜDHOFF, H., HAHN, T., JUNGBLUT, P.W., KALLWEIT, E., CALVETE, J.J., ENSSLIN, M., HOPPEN, H.O., WINTERGALEN, N. & WEITZE, K.F., 1995. Advanced ovulation in gilts by the intrauterine application of a low molecular mass pronase-sensitive fraction of boar seminal plasma. J Reprod Fertil., 105: 247-252.
ZOU, C.X. & YANG, Z.M., 2000. Evaluation on sperm quality of freshly ejaculated boar semen during in vitro storage under different temperatures. Theriogenology, 53: 1477-1488.
BERGER, T. & CLEGG, E.D., 1985. Effect of male accessory gland secretions on sensitivity of porcine sperm acrosomes to cold shock, initiation of motility and loss of cytoplasmic droplets. J Anim Sci., 60: 1295-1302.
BONET, S., BRIZ, M. & FRADERA, A., 1993. Ultrastructural abnormalities of boar spermatozoa. Theriogenology, 40: 383-396.
CABALLERO, I., 2007. Estudio del plasma seminal y la espermadhesina PSP-I/PSP-II sobre la funcionalidad de los espermatozoides de verraco: Tesis, Universidad de Murcia, Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, España.
CABALLERO, I., VÁZQUEZ, J.M., GIL. M.A., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M., RODRÍGUEZ-MARTÍNEZ, H. & MARTÍNEZ, E.A., 2004. Does seminal plasma psp-i/psp-ii spermadhes in modulate the ability of boar spermatozoa to penetrate homologous oocytes in vitro? J Androl., 25: 1004-1012.
CALVETE, J.J., DOSTÁLOVÁ, Z., SANZ, L., ADERMANN, K., THOLE, H.H., TÖPFER-PETERSEN, E., 1996. Mapping the heparin-binding domain of boar spermadhesins. FEBS Letters, 379: 207-211.
CALVETE, J.J., ENSSLIN, M., MBURU, J., IBORRA, A., MARTÍNEZ, A., ADERMANN, K., WABERSKI, D., SANZ, L., TÖPFER-PETERSEN, E., WEITZE, K.F., EINARSSON, S. & RODRÍGUEZ-MARTÍNEZ, E., 1997. Monoclonal antibodies against boar sperm zonapellucida-binding protein awn-1. Characterization of a continuous antigenic determinant and immunolocalization of awn epitopes in inseminated sows. Biol Reprod., 57: 735-742.
CENTURIÓN, F., VÁZQUEZ, J.M., CALVETE, J.J., ROCA, J., SANZ, L., PARRILLA, I., GARCÍA, E.M. & MARTÍNEZ, E.A., 2003. Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution. Biol Reprod., 69: 640-646.
EKHLASI-HUNDRIESER, M., SINOWATZ, F., GREISER DE WILKE, I., WABERSKI D. & TÖPFER-PETERSEN, E., 2002. Expression of spermadhesin genes in porcine male and female reproductive tracts. Mol Reprod Dev., 61 (1): 32-41.
FISCHER, K.A., LEYEN, K.V., LOVERCAMP, K.W., MANANDHAR, G., SUTOVSKY, M., FENG, D., SAFRANSKI, T. & SUTOVSKY, P., 2005. 15-lipoxygenase is a component of the mammalian sperm cytoplasmic droplet. Reproduction, 130: 213-222.
FOLEY, C.W., HEIDENREICH, C.J., HARRINGTON, R.B., JONES, H.W. & ERB, R.E., 1964. Changes in fructose, lacticacid, ph, and motility of boar semen during incubation at 37°C. J Anim Sci., 23: 558-561.
GARCÍA, E.M., 2007. Análisis, función y aplicaciones biotecnológicas de las proteínas del plasma seminal de porcino PSP I y PSP II: Tesis, Universidad de Murcia, Facultad de Veterinaria, Departamento de Medicina y Cirugía Animal, España.
GARCÍA, J.C., DOMÍNGUEZ, J.C., PEÑA, F.J., ALEGRE, B., GONZÁLEZ, R., CASTRO, M.J., HABING, G.G. & KIRKWOOD, R.N., 2010. Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility. Anim Reprod Sci., 119: 160-165.
GARNER, D.L., THOMAS, C.A., GRAVANCE, C.G., MARSHALL C.E., DEJARNETTE, J.M. & ALLEN, C.H., 2001. Seminal plasma addition attenuates the diltion effect in bovine sperm. Theriogenology, 56: 31-40.
GÓMEZ, G., 2010. Dinámica de la calidad seminal de verracos del centro-occidente colombiano: Tesis, Universidad de Caldas, Maestría en Sistemas de Producción Agropecuaria, Manizales, Colombia.
HARAYAMA, H., SHIBUKAWA, T., MIYAKE, M., KANNAN, Y. & KATO, S., 1996. Fructose stimulates shedding of cytoplasmic droplets from epididymal boar spermatozoa. Reprod Fertil Dev., 8: 1039-1043.
JONÁKOVÁ, V. & TICHÁ, M., 2004. Boar seminal plasma proteins and their binding properties. A review Collection of Czechoslovak Chemical Communications, 69: 461-475.
KAPLAN, M., RUSSELL, L.D., PETERSON, R.N. & MARTAN, J., 1984. Boar sperm cytoplasmic droplets: Their ultrastructure, their numbers in the epididymis and at ejaculation and their removal during isolation of sperm plasma membranes. Tissue and Cell, 16: 455-468.
KATO, S., SHIBUKA, W.A.T., HARAYAMA, H., et al., 1996. Timing of shedding and dissentegration of cytoplasmic droplet from boar and goat spermatozoa. J. Reprod. Dev., 42: 237-241.
KAWANO, N., SHIMADA, M. & TERADA, T., 2004. Motility and penetration competence of frozen-thawed miniature pig spermatozoa are substantially altered by exposure to seminal plasma before freezing. Theriogenology, 61: 351-364.
LOVERCAMP, K.W., SAFRANSKY, T.J. & FISCHER, K.A., 2007. High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters. Arch Androl, 53: 219-228.
OKAMURA, N., DACHEUX, F., VENIEN, A., ONOE, S., HUET, J.C. & DACHEUX, J.L., 1992. Localization of a Maturation-Dependent Epididymal Sperm Surface Antigen Recognized by a Monoclonal Antibody Raised against a 135-Kilodalton Protein in Porcine Epididymal Fluid. Biol Reprod., 47: 1040-1052.
PÉREZ-LLANO, B., LORENZO, J.L., YENES, P., TREJO, A. & GARCÍA-CASADO, P. 2001. Short hypoosmotic swelling test for the prediction of boar sperm fertility. Theriogenology, 56: 387-398.
PURSEL, V.G. & JOHNSON, L.A., 1974. Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Theriogenology, 1: 63-68.
RODRÍGUEZ-MARTÍNEZ, H., SARAVIA, F., WALLGREN, M., TIENTHAI, P., JOHANNISSON, A., VÁZQUEZ, J.M., MARTÍNEZ, E., ROCA, J., SANZ, L. & CALVETE, J.J., 2005. Boar spermatozoa in the oviduct. Theriogenology, 63:514-535.
ROZEBOOM, K.J., 2000. Evaluating of boar semen quality (en) N. C. S. UNIVERSITY (ed.) Animal Science Facts. Extension Swine Husbandry, North Carolina State, 1-8.
SALA-ECHAVE, R., REGUERA, G., GARCÍA-CASADO, P. & PÉREZ-LLANO, B., 2008. Influencia del calentamiento de las dosis seminales porcinas sobre la calidad espermática durante la I.A. Av. Tecnol. porc., 5 (3): 34-41.
SARAVIA, F., WALLGREN, M., JOHANNISSON, A., CALVETE, J.J., SANZ, L., PEÑA, F.J., ROCA, J. & RODRÍGUEZ-MARTÍNEZ, H., 2009. Exposure to the seminal plasma of different portions of the boar ejaculate modulates the survival of spermatozoa cryopreserved in MiniFlatPacks. Theriogenology, 71: 662-675.
WABERSKI, D., SÜDHOFF, H., HAHN, T., JUNGBLUT, P.W., KALLWEIT, E., CALVETE, J.J., ENSSLIN, M., HOPPEN, H.O., WINTERGALEN, N. & WEITZE, K.F., 1995. Advanced ovulation in gilts by the intrauterine application of a low molecular mass pronase-sensitive fraction of boar seminal plasma. J Reprod Fertil., 105: 247-252.
ZOU, C.X. & YANG, Z.M., 2000. Evaluation on sperm quality of freshly ejaculated boar semen during in vitro storage under different temperatures. Theriogenology, 53: 1477-1488.
